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投稿时间：2018-07-14
投稿时间：2018-07-14
中文摘要: 以棘孢木霉（Trichoderma asperelham）为真空冷冻干燥菌种，以菌种存活率、椪柑橘瓣降解时间为评价指标，通过单因素试验研究保护剂添加量、菌液浓度和预冻时间对棘孢木霉真空冷冻干燥效果的影响，在此基础上，采用正交试验优化其真空冷冻干燥工艺，结果表明最佳工艺条件为：保护剂添加量为20%、菌液浓度为1×108个/mL、预冻时间为24 h，在此工艺条件下菌株的存活率达到81.25%，橘瓣降解时间最低达到31 min。工艺优化后使得菌种存活率和菌株活性都保持较高水平，其发酵液降解橘瓣所需时间较短，该工艺可用于棘孢木霉的冻干处理。
Abstract:In this experiment，Trichoderma asperelham was used as bacterial species of vacuum freeze drying，servival rate and degradation time of Ponkan segments were used as evaluating indicator，the content of cryoprotectant，concentration of bacteria solution and pre-freezing time were probed via single factor experiments.On the basis of this，the optimal vacuum freeze drying conditions were achieved by orthogonal experiment as follows：content of cryoprotectant 20%，concentration of bacteria solution 1×108cells/mL and pre-freezing time 24 h，servival rate reached the maximum value 81.25%，and degradation time of Ponkan seg-ments reached the minimum value 31 min under the optimum conditions.The survival rate and activity of the optimized process bacteria were high，and the time required for degradation was short.This process can be used for the vacuum freeze drying treatment of Trichoderma asperelham.
keywords: Trichoderma asperelham vacuum freeze drying servival rate degradation time orthogonal experiment
文章编号：201902027 中图分类号： 文献标志码：
基金项目:湖南省果蔬清洁加工工程技术研究中心（2015TP2022）；湖南省自然科学基金青年项目（2017JJ3284）；湖南省科技厅“三区”科技人才项目（S2017NCTPSQ0044）；湖南省教育厅科学研究一般项目（17C1433）和优秀青年项目（17B241）；邵阳学院研究生科研创新项目（CX2017SY017）
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