Abstract:An analytical method for determination of four selenium species in selenium-enriched wheat by high performance liquid chromatography-inductively coupled plasma mass spectrometry （HPLC-ICP-MS）was established.Selenite（SeIV），selenate（SeVI），selenomethionine（SeMet）and selenocystine（SeCys2）were quantitatively extracted with Protease XIV in a microwave field.The results showed that four selenium species could be separated within 9 min by using a Hamilton PRP X-100 reversed-phase anion exchange column with 6 mmol/L citric acid as mobile phase under pH 5.0.The detection limits of SeCys2，SeIV，SeMet，SeVI were 0.23，0.15，0.30，0.16 μg/L with correlation coefficient（r2）of more than 0.999，the recoveries were in the range of 93.7%-105.2%，and the relative standard deviations（RSD）were 2.2%-6.6%（n=6）.The proposed method has some advantages including good accuracy，high precision，fast and simple operation，which can meet the requirements for analyzing of selenium species in selenium-enriched wheat.