Abstract:This paper adopted enzymolysis to extract can oil from almond residue，through the single element experiment，we definited that the material's granularity was 100，the mixed-enzymes of neutral protease and cellulase （1∶1）was the extracted enzyme.The amount of enzyme，the enzymolysis pH，the enzymolysis time and the enzymolysis temperature were chosen as the main factors based on the single element and L9（34）orthogonal experiment were optimized.The best condition for extraction by the orthogonal experiment was summarized：the amount of enzyme was 3%，the enzymolysis pH was 7.5，the enzymolysis temperature was 40℃，the enzymolysis time was 2.5 h，the oil yield was 26.25%.The quality of the reliquus oil was yellow，diaphanous and had strong almond flavor.