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投稿时间：2018-06-28
投稿时间：2018-06-28
中文摘要: 用复合酶（碱性蛋白酶、木瓜蛋白酶）水解方法制备葡萄籽多肽，通过单因素试验考察酶添加量、酶解时间、酶解温度和酶解pH值对脱脂葡萄籽蛋白水解度的影响。在基于单因素试验的条件下，选用三因素三水平试验确定复合酶分析3个因素对响应值的影响。结果表明最佳酶解工艺参数为：复合蛋白酶质量比例为7∶3，酶添加量为2%、酶解时间3 h、酶解温度50℃、pH 7.4，在该条件下脱脂葡萄籽蛋白的水解度达24.33%，葡萄籽蛋白复合蛋白酶酶解物在质量浓度为120 μg/mL～600 μg/mL的范围内，其对DPPH自由基的清除能力为19.25%～41.03%，且存在明显的量效关系，与VC、VE相比，葡萄籽多肽用量为0.15%时抗氧化效果较好。
中文关键词: 酶解法 脱脂葡萄籽多肽 提取工艺 响应面分析 DPPH自由基清除率
Abstract:Degreased grape seed peptides were prepared by compound protease hydrolysi（alcalase and papain respectively）.The effects of enzyme addition，enzymolysis time，enzymolysis temperature and enzymolysis pH on the degree of hydrolysis of degreased grape seed peptide were investigated by single factor experiments.On the basis of single factor experiments，three factors and three levels were selected to analyze by the response surface method.The results showed that the optimum enzymolysis parameters were as follows：the mass ratio of alcalase to compound protease was 7 ∶3，the enzyme adding amount was 2%，enzymolysis time was 3 h，enzymolysis temperature was 50℃，pH was 7.4，under these condition，the degree of hydrolysis degreased grape seed peptide was 24.33%.The scavenging ability of DPPH free radicals free radicals was detectde，and DPPH scavenging ratio were 19.25%-41.03%with hydrolysates concentration of 120 μg/mL-600 μg/mL and presented a quantity-effect relation，compared with VCand VE，the antioxidant effect of grape seed polypeptide was better when the dosage was 0.15%.
keywords: enzymatic hydrolysis degreased grape seed peptide extraction process response surface analysis DPPH free radicals scavenging ratio
文章编号：201901022 中图分类号： 文献标志码：
基金项目:兵团科技攻关与转化项目（2016AB009）
| 作者 | 单位 |
| 李文慧，刘飞，刘江，赵巧玲，董娟 | 1.石河子大学食品学院，新疆石河子832000；2.喀什大学生命与地理科学学院，新疆喀什844006；3.新疆赛里木现代农业股份有限公司，新疆博乐833400 |
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