Abstract:An analytical method was developed for the simultaneous determination of 28 pesticide in vegetables based on solid phase extraction followde by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）.The samples were extracted using acetonitrile homogenate and centrifuged by salting，NH2solid phase extraction column purification.The target analytes were separated on HPLC column and analyzed in multiple reaction monitoring（MRM）mode.Sample matrix-matched calibration was used to determine the residue contents by external standard.The results showed that the calibrationcurves in the range of 7.5 ng/mL-300 ng/mL for acetamiprid，and 5.0 ng/mL-200 ng/mL for other pesticides，with correlation coefficients larger than 0.996 8.The limits of quantitation （LOQ）were of 28 pesticides 0.7 μg/kg-6.0 μg/kg.The recoveries of acetamiprid with the concentration levels at 30，60，150 μg/kg and other pesticides with the concentration levels at 20，40，100 μg/kg were range of 63.9%-120.4%，which relative standard deviations （RSD）were 2.4%-9.8%.This method was suitable for qualification of 28 pesticide in vegetables，meet the daily testing requirements of the laboratory and has practical application significance.