Abstract:The contents determination method of rutin，quercetin，kaempferol and kaempferol-3-o-rutinoside in buckwheat was established，and the content difference of flavonoids in buckwheat from different areas was compared.High performance liquid chromatography （HPLC） was adopted for content determination of flavonoids in buckwheat from different areas.Statistical method was used to analyze the differences among the flavonoids from different areas.The linear range，precision，stability，repeatability and recovery test in the methodology are all in accordance with the regulations.The contents of quercetin and kaempferol in buckwheat from Yunnan areas were significantly higher than other areas，rutin and kaempferol-3-o-rutinoside was not different.The method can be used for the contents determination of flavonoids in buckwheat.This study can provide technical basis for the purchase of buckwheat raw materials.