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投稿时间：2018-05-23
投稿时间：2018-05-23
中文摘要: 建立硅胶柱层析法分离制备栀子黄色素中藏红花素I的方法，并对所得产品进行抗氧化性与稳定性研究。建立了乙酸乙酯-甲醇-水（体积比为100∶20∶15至100∶25∶15）的梯度洗脱体系，以及层析柱硅胶与栀子黄色素的质量比为350∶1的上样量比例，并制备出纯度为98.39%的藏红花素I单体样品，其得率为15.58%，回收率为50.42%。抗氧化与稳定性研究结果表明，藏红花素I具有优良的抗氧化活性；藏红花素I在高温、强光照射、强酸强碱环境中均会产生损失。含藏红花素I成分的产品应贮存于低温、避光、pH值为6的环境中，且与适量抗坏血酸联用可以提高其稳定性。
Abstract:To establish a method for the separation and preparation of crocin I from gardenia yellow pigment by silica gel column chromatography，and to study the antioxidation and stability of the obtained product.Established a gradient elution system was ethyl acetate-methanol-water（100∶20∶15 to 100∶25∶15 by volume）and the optimal proportion of the loading amount was the mass ratio of silica gel to gardenia yellow pigment with 350 ∶1，and a 98.39%purity sample of crocin I was prepared with a yield of 15.58%and a recovery rate of 50.42%.The results of antioxidant and stability studies showed that crocin I had excellent antioxidant activity，and crocin I was lost in high temperature，strong light，strong acid and alkali environment.The products containing crocin I should be stored in an environment with low-temperature，light-proof，pH 6，and can be used in combination with an appropriate amount of ascorbic acid to improve its stability.
keywords: gardenia yellow pigment crocin I silica gel column chromatography stability in vitro antioxidation
文章编号：201824011 中图分类号： 文献标志码：
基金项目:
| 作者 | 单位 |
| 陈哲，赵庆生，秦雯，赵兵，葛思琪，赵强，查圣华 | 1.北京城市学院，北京100094；2.中国科学院过程工程研究所，生化工程国家重点实验室，北京100190；3.南阳泰瑞生物科技股份有限公司，河南南阳473412 |
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