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食品研究与开发:2018,39(23):134-141
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食品接触材料中3种致病菌的多重荧光PCR检测
柯振华,张雅薇,陈筱婷,林碧莲,孟鹏,高宇,戴明
((福建省产品质量检验研究院国家加工食品质量监督检验中心(福州),福建福州350000))
Multiplex Real-Time PCR Assays for Detection of Three Pathogenic Bacteria in Food Contact Material
KE Zhen-hua,ZHANG Ya-wei,CHEN Xiao-ting,LIN Bi-lian,MENG Peng,GAO Yu,DAI Ming
((National Centre for Quality Supervision and Testing of Processed Food(Fuzhou),Fujian Inspection and Research Institute for Product Quality,Fuzhou 350000,Fujian,China))
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投稿时间:2018-08-20    
中文摘要: 建立并优化食品接触材料中金黄色葡萄球菌、沙门氏菌和副溶血性弧菌3种致病菌的多重实时荧光聚合酶链式反应(polymerase chain reaction,PCR)检测方法,评价此方法的特异性和灵敏度,并模拟阳性样品进行检测。结果显示,该方法特异性良好,检测灵敏度均能达到103CFU/mL,为食品接触材料的微生物检测提供技术保障。
Abstract:Abstract:Multiplex real-time polymerase chain reaction(PCR)assays were established and optimized for detection of Staphylococcus aureus,Salmonella and Vibrio parahaemolyticus in food contact materials.The specificity and sensitivity of this method was evaluated and the simulated positive samples are tested.The results showed that this method showed good specificity and the sensitivity could achieve to 103CFU/mL,which provided technical support for detection of pathogenic bacteria in food contact material.
文章编号:201823024     中图分类号:    文献标志码:
基金项目:福建省质量技术监督局科技项目(FJQI2016010)
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