本文已被：浏览 1364次 下载 0次
投稿时间：2018-03-30
投稿时间：2018-03-30
中文摘要: 以玫瑰花为原料，采用响应面法优化玫瑰多糖提取工艺，并对玫瑰花多糖结构及其体内抗氧化活性进行研究。结果表明：最优的提取工艺条件为提取温度为95℃，提取时间为256 min，液料比30∶1（mL/g），玫瑰花多糖得率为1.46%。紫外光谱图表明玫瑰化多糖中不含蛋白质和核酸，红外光谱显示，在3 433、2 928、1 345 cm-1等处都有很明显的多糖的特征吸收峰。抗氧化试验结果表明：玫瑰花多糖可显著提高衰老小鼠血清和肝组织中超氧化物歧化酶（superoxide dismutase，SOD）和谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力（P＜0.05），显著降低丙二醛（malondialdehyde，MDA）含量（P＜0.01），表现出良好的体内抗氧化活性。
Abstract:The paper chooses Rosa rugosa Thunb.Petals as material.We conduct a series of studies about polysaccharide of Rosa rugosa Thunb.Petals（RRPP）about the optimization of the extraction process，preliminary structural analysis，and its antioxidant activity.The conclusions were as follows：the optimal extraction conditions for the extraction temperature：95 ℃，extraction time：256 min，liquid-solid ratio was 30 ∶1 （mL/g）.Under these conditions，the RRPP extraction rate was 1.46%.Ultraviolet sepectrum analysis showed not contains nucleic acids and proteins. Infrared spectrum displayed that theRRPP have the characteristic absorption peaks（3433cm-1，2 928 cm-1and 1 345 cm-1）of polysaccharides.The antioxidant ability results showed that the activities of superoxide dismutase（SOD），and glutathione peroxidase（GSH-Px）in serum and liver tissues of the aging mice treated with RRPP significantly enhanced comparing with control mice （P＜0.05），the contents of malondialdehyde（MDA） were also detected to be significantly reduced（P＜0.01）.So reach the conclusion that RRPP possessed a strong antioxidant activity in vivo.
keywords: Key words：response surface methodology Rosa rugosa Thunb.petals polysaccharides structure analysis antioxidant ability
文章编号：201822008 中图分类号： 文献标志码：
基金项目:黑龙江省高校基本科研业务费基础研究项目（2016-KYYWF-0675）
引用文本：
津ICP备20000603号-1