Abstract:The gangliosides in pig brain tissue extract was purified by method of slica column chromatography.Based on single factor experiments and response surface methodology，the optimum conditions were as follows：the sample that gangliosides concentration of crude extract was 7.75%was loaded on the column which filled with 34.89 g slica at flow rate of 2.5 BV/h，then the eluent contained ethyl acetate and methyl alcohol，which volume ratio was 4 ∶1，washed the colume at flow rate of 2.0 BV/h，and the gangliosides in product was analysed.The recovery of gangliosides in final product was 86.9%，and the content has increased 2.89 times from the rate before purification.Therefore，the process is convenient and feasible.