Abstract:The antioxidant activities of total flavonoids from Osmanthus fragrans（TFOF）in vitro were evaluated by DPPH·and ABTS+·scavenging assay and the potassium ferricanide reduction methods.The aging mouse model induced by subcutaneous injection of D-galactose were the subject investigated in vivo to evaluate the effect of TFOF on the antioxidant capacity of the body.The results showed that in vitro experiments TFOF could effectively scavenge DPPH· and ABTS+·，and showed the reducing capacity on ferricyanide.And in vivo test，compared with the blank group，the malondialdehyde（MDA）contents in serum，liver and kidney homogenate of the model group were increased，and the contents of superoxide dismutase（SOD），glutathione peroxidase（GSH-Px）and glutathione （GSH）in liver and kidney were decreased（P＜0.05），showed the animal aging model was successfully established by injecting D-galactose；compared with the aging group the SOD、GSH、GSH-Px content of serum、liver and kidney was increased in high dose group （P＜0.05），and the content of MDA in all experiment groups was decreased（P＜0.05）.The TFOF could improve the cell shape and structure of D-gal aging mice's liver cells significantly.Our research showed that the total flavonoids from Osmantus fragrans have good antioxidant activity by scavenging free radicals and reducing oxidative damages.