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食品研究与开发:2018,39(6):172-177
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几株适用于发酵肉制品的乳酸菌的分离筛选及鉴定
李建1,李丽1,彭翠珍1,罗碧霞1,曾雄1,宗绪岩1,2,*
(1.四川理工学院 生物工程学院,四川自贡643000;2.酿酒生物技术及应用四川省重点实验室,四川自贡643000)
Selection and Identification of Several Lactic Acid Bacteria Strains for Fermented Meat Products
LI Jian1,LI Li1,PENG Cui-zhen1,LUO Bi-xia1,ZENG Xiong1,ZONG Xu-yan1,2,*
(1.College of Bioengineering,Sichuan University of Science&Engineering,Zigong 643000,Sichuan,China;2.Sichuan Provincial Key Lab of Liquor-marking Biotech&Application,Zigong 643000,Sichuan,China)
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投稿时间:2017-12-04    
中文摘要: 为获得适合肉制品发酵的优良乳酸菌菌株,运用经典乳酸菌筛选法与分子生物学相结合方法从自然发酵香肠及泡菜水中分离纯化乳酸菌,并对其进行产气、产黏液等发酵特性测定试验,结合各菌株产酸能力以及在不同温度、pH、NaCl、NaNO2的条件下的生长情况,最终筛选得到4株符合要求的乳酸菌。经形态学和16S rDNA序列分析,结果表明其中三株为植物乳杆菌(XC-3、XC-5、XC-9),另一株为鼠李糖乳杆菌(PC-3)。
中文关键词: 发酵香肠  泡菜水  乳酸菌  分离  鉴定
Abstract:In order to screen out high-quality lactic acid bacteria starter adapting to fermented meat products,using natural fermented sausages and brine as the research object,lacticacid bacteria were isolated and purified by Classical Lactobacillus screening method ,and it was identified by 16S rDNA sequencing technique.The fermentation characteristics of different lactic acid bacteria had also been studied,such as viscosity producing and gas-generation,etc.Combined with the acid producing ability and growth under different temperatures,pH,NaCl Concentration,NaNO2Concentration,a total of 4 strains of lactic acid bacteria were screened.By morphological and 16S rRNA sequence analysis,among the 4 representative strains,3 strains were Lactobacillus plantarum(XC-3,XC-5,XC-9),1 strain was Lactobacillus rhamnosus(PC-3).
文章编号:201806032     中图分类号:    文献标志码:
基金项目:固态酿造关键技术研究四川省院士(专家)工作站开放基金项目(GY2014-01);四川理工学院创新基金资助项目(y2016004);四川省大学生创新创业训练计划项目(201510622058);四川省教育厅科技项目(15ZA0233);香料植物资源开发与利用四川省高校重点实验室开放基金项目(2015XLY007)
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