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投稿时间：2017-08-10
投稿时间：2017-08-10
中文摘要: 建立婴幼儿米粉中硝酸盐和亚硝酸盐的在线超滤-离子色谱测定方法。样品经超声提取，3%乙酸沉淀蛋白质后，低温离心，取上清液依次过C18小柱，Ag柱、Na柱，经在线超滤装置自动净化样品，由电导检测器检测样品中的硝酸盐和亚硝酸盐含量。硝酸盐（以NO3-计）和亚硝酸盐（以NO2-计）分别在0.10 mg/L~2.0 mg/L和0.010 mg/L~0.20 mg/L的范围内线性良好，相关系数R2均大于0.999 9。方法的硝酸盐（以NO3-计）检出限为0.2 mg/kg，亚硝酸盐（以NO2-计）检出限为0.1 mg/kg，硝酸盐回收率为89.8%~96.1%，亚硝酸盐回收率为89.5%~96.0%。此法所获得结果准确可靠，重复性良好，实现对婴幼儿米粉中硝酸盐和亚硝酸盐的准确定量分析。
Abstract:A method was established for the determination of nitrate and nitrite in baby rice flour by ion chromatogrephy-inline ultraflitration.After ultrasonic extraction，protein precipitation using 3%acetic acid，and centrifugaion at low temperature，the supernatant was filtered through C18column，Ag column and Na column for injection analysis.And then it was analyzed by Ion chromatogrephy-inline ultraflitration，and the conductivity dectector was used to detect nitrate and nitrite in baby rice flour.The lineal range of nitrate in 0.10 mg/L-2.0 mg/L and the lineal range of nitrite in 0.010 mg/L-0.20 mg/L was with high correlation coefficients（R2＞0.999 9）.This method detection limit was 0.2 mg/kg for nitrate（based on NO3-），0.1 mg/kg for nitrite（based on NO2-）.The recovery rate of nitrate was 89.8%-96.1%，and the recovery rate of nitrite was 89.5%-96.0%.This method was accurate，reproducible，and suitable for determination of nitrate and nitrite in baby rice flour.
文章编号：201804028 中图分类号： 文献标志码：
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