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投稿时间：2017-08-19
投稿时间：2017-08-19
中文摘要: 建立高效液相色谱（High Performance Liquid Chromatography，HPLC）法对橙子中L-抗坏血酸进行含量测定研究。20 g/L偏磷酸溶液作为橙子中L-抗坏血酸的提取剂，超声5 min提取。液相色谱柱采用Inertsil ODS-3C18（5 μm，4.6 mm×250 mm），柱温 25℃，0.05 mol/L磷酸溶液-甲醇（90∶10）作为流动相，流速0.800 mL/min，检测波长 245 nm。L-抗坏血酸标准品以不同浓度进样，获得标准曲线Y=60 408.6x+38 583.7，相关系数R2=0.999 6；在1 μg/mL～50 μg/mL范围内L-抗坏血酸有良好的线性关系；检出限为0.5 μg/mL；精密度相对标准偏差RSD=0.67%。
Abstract:A method for the determination of L-ascorbic acid in orange by HPLC was established.20g/L metaphosphoric acid solution was used as the extraction agent of L-ascorbic acid in orange and ultrasonic extracted for 5 minutes.The chromatographic separation was performed on an Inertsil ODS-3C18column（5 μm，4.6 mm×250 mm），at 25℃using 0.05 mol/L phosphoric acid solution-methanol（90∶10）as the mobile phase at a flow rate of 0.800 mL/min and the detection wavelength was 245 nm.Different volume of sodium L-ascorbic acid standard were injected，the standard curve was Y=60 408.6 x+38 583.7，with a correlation coefficient of 0.999 6.It showed good linearity in the concentration range of 1 μg/mL to 50 μg/mL.The detection limit was 0.5 μg/mL.The relative standard deviation of the precision was 0.67%.
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