Abstract:This study aimed to establish a real-time fluorescence loop-mediated isothermal amplification（RFLAMP）assay to detect Yersinia enterocolitica （Y.enterocolitica）.The products were identified by restriction digestion followed by electrophoresis and the results indicated that the amplification was correct.The performance of the assay was evaluated with respect to specificity and sensitivity，and the detection limit of artificially contaminated chicken was tested.Among the strains tested，Y.enterocolitica strain was identified as positive，the other non-Y.enterocolitica strains were negative.The sensitivity for Y.enterocolitica was 61 CFU/mL in pure cultures.The detection limit for artificially contaminated chicken was 46 CFU/g.This method has high specificity and sensitivity and allowed real-time monitoring，which was useful to realize rapid detection of Y.enterocolitica.