Abstract:In this paper the optimum extraction conditions of polysaccharides extracted by hot water from Pholiota nameko were determined by single-factor test and orthogonal test.According extracting temperature，extracting time and ratio of water to Pholiota nameko were influencing on extraction rate of polysaccharides，the optimum extraction parameters of experiment were as follows：temperature was 85℃，time was 2 h，ratio of water to Pholiota nameko was 1 ∶40 （g/mL）.By using this condition test，Pholiota nameko polysaccharide reached 24.55%.