Abstract:The chicken blood as the fermented materials，the expanding culture conditions for composite strains was studied and optimized by response surface.The temperature，pH and rotational speed were as the factors，based on the OD value of bacteria concentration as the single factor index.According to the design principle of Box Behnken（BBD）in the center combination，three factors and three levels experiments were designed to obtaintheoptimumfermentedconditionsforthemixedstrains：temperature30℃，pH=7，rotationalspeed140r/min，under these optimal conditions，the OD value reached the maximum.This experiment provides scientific research and application value for further research and development of animal by-products.