Abstract:Fingerprint of amino acids in honey was constructed by capillary electrophoresis.Herein，bacterial cellulose（BC）was used as additive in running buffer to improve the separation efficiency.Experimental parameters such as the pH and concentration of the running buffer，separation voltage as well as content of BC in running buffer were systematically investigated.The results showed that 17 amino acids derivatized with fluorescein isothiocyanate（FITC）could be baseline separated within 25 min using Na2B4O7-NaH2PO4buffer（30 mmol/L，pH 9.8）containing 0.5%BC for background electrolyte，25 kV for the separation voltage.Under the optimized conditions，17 amino acid derivatives showed good linearities with correlation coefficients greater than 0.993.The limit detections of this developed method for 17 amino acid derivatives were ranging from 2.5×10-4to 30.0×10-4μmol/L.The relative standard deviations（RSDs）for peak areas were 1.84%-5.50%（inter-day）and 0.94%-3.10% （intra-day），respectively.The developed method was further applied to determine the amino acids in Acacia honey with the satisfactory recovery in the range of 81.10%-96.15%with RSDs（n=3）below 8.22%.