Abstract:With Ginger protease as the research object，on the basis of single factor experiment，the enzyme activity determination conditions were optimized by the response surface，the effects of reaction temperature，reaction time，substrate concentration，buffer pH effects on Ginger enzyme activity.The results showed that Ginger protease activity determination of optimum condition：reaction temperature 50℃，the reaction time was 3.6 min，the substrate concentration 0.95%，buffer pH6.78.Measured under the conditions of Ginger protease activity of 130.46 U.The result of verification experiment was coincided with predictive value，the actual activity was 133.15 U.The results showed that it was feasible to optimize the determination of Ginger protease activity by response surface methodology.