Abstract:Genomic DNA of 8 strains of bacteria and 4 strains yeasts，isolated from Sichuan pickles，was extracted by using an alkaline lysis method.Concentration and purity of strain DNA extracts，quality of PCR amplified products，results of nucleotide sequencing and strain identification were studied.Furthermore，the cost and time-consuming of this method were evaluated.Results showed that the DNA concentration and（A260/A280）of the extracts were all>40 ng/μL and>1.9，respectively.It suggested good quality of the DNA extracts.12 strains isolated from Sichuan pickles were identified into species level，and their results were consistent with that obtained from test kit assays，which indicates a high accuracy of the method.The cost and time-consuming of the method were evaluated as fifty yuan per 100 times and 50 min，respectively.And so，it was much better than the test kit assays used currently.