Abstract:Using the fruits of Physalis alkekengi as the research object，polyphenols was extracted using alcohol.Nine macroporous resins were selected to purify polyphenols of the fruits of Physalis alkekengi and the optimal purification conditions，and its antioxidant activity and inhibition of enzyme activity were determined by using chemical methods.The results showed that the AB-8 macroporous resin had the best purification effect；and the optimal purification conditions were as follows：polyphenols concentration in a loading solution was 0.3 mg/mL；the optimal pH value was the pH value of the sample itself；the flow rate of the column was 1 mL/min；the ethanol concentration was 80%；and the elution flow rate was 1 mL/min.The experimental results showed that the Physalis fruit polyphenols had a certain reducing capacity on·OH and DPPH·，with the scavenging IC50values were 0.604 4 mg/mL and 2.262 mg/mL.The IC50for inhibiting activity against pancreatic lipase，α-amylase and α-glucosidase were 0.8262 mg/mL，0.817 8 mg/mL and 1.011 mg/mL，respectively.The results demonstrated that the Physalis fruit polyphenols have antioxidant activity and inhibitory effects against pancreatic lipase，α-amylase and α-glucosidase in vitro.