Abstract:Comparing five methods to extract anthocyanins，it was found that the extraction rate of anthocyanins in black bean hulls was the highest by ethanol/ammonium sulfate aqueous two phase system.The optimum conditions for the extraction of aqueous two phase were determined by single factor test.The response surface methodology was used to optimize the extraction of anthocyanins from black soybean hull by aqueous two phase.The optimal parameters were optimized by response surface analysis.The liquid ratio，ammonium sulfate mass fraction，ethanol concentration，pH were 1∶56（g/mL），22%，30%，3.0 respectively.The optimal yield of anthocyanin was 2.81 mg/g.The research will provide theoretical support for the anthocyanin of practical application.