Abstract:In order to enrich more types of leavening agents and probiotics resources，faeces collected from seven infants（Alashan，Inner Mongolia）were investigated.On the basis of traditional bacterial separation method，Mupirocin lithium salt and X-Gal were added to the basic medium，to separate the bifidobacterium effectively.Automatic microorganism analytical system and API20A reagent strip were applied to identify the bifidobacterium.In total，six strains of bifidobacterium were obtained from infacts'faeces，among which three strains were Bifidobacterium lactis，two strains were Bifidobacterium adolescentis，and one strain was Bifidobacterium breve，and the optimum storage condition for the bifidobacterium is 70%glycerol.