Abstract:Indigenous Saccharomyces cerevisiae KDLYS9-3 produces high activity beta-D-glucosidase，and it has the effect of enhancing aroma during the wine making process.Based on the experiment of bacteria growth and enzyme production，the Logistic equation，DoseResp equation and Nelder equation were used to build a dynamic model for the yeast growth and enzyme production，and the relationship of yeast growth rate and enzyme producing rate in this study.After the nonlinear fitting by Origin 8.0 software，and graphed by the method of Lineweaver-Burk，the kinetic parameters of Kmand Vmaxvalues for the enzymes of this yeast producing beta-D-glycosidase were detected.The result shows that the bacteria growth and enzyme production dynamic model of KDLYS9-3 were correlation with the test results，the dynamic model equation can reflect the variety regulation of bacteria growth and enzyme production，the correlation was part of the coupling relationship.The yeast began to produce enzymes after 8 h growth，and it produced a large number of enzymes when entered the logarithm of growth.The growth of yeast was stable after 39 h.With the decline phase of the yeast，the enzyme also stopped producing.The kinetic parameters of the KDLYS 9-3 enzyme were obtained by the double reciprocal method.The Kmwas 8.492 579 mmol/L，and the Vmaxwas 1.030 715 （mu/L）/min.The results helped set the basement of the strains'theoretical foundation and practical application.