Abstract:Gibel carp （Carassius auratus gibelio）protein was chosed as the raw material.It was independently hydrolyzed by trypsin，pepsin，flavourzyme，neutrase，alcalase，papain，bromelain and acid protease.The antioxidant capability was evaluated by the DPPH free radicals clearance rate.The results showed that the flavourzyme was the best proteolytic enzyme.The single factor and orthogonal experiment showed that the best hydrolysis conditions of the flavourzyme were：enzyme amount 3 000 U/g，substrate concentration 7%，pH=7.5，T=55℃，reaction time 4 h.Under these conditions，the degree of hydrolysis（DH）was 46.78%，the DPPH free radicals clearance rate could achieve to 90.12%，the scavenging rate of hydroxyl radical was 82.31%and the clearance rate of superoxide anion radical was 79.45%.The enzymatic hydrolysates were processed with ammonium sulfate salting-out，active carbon，ultrafiltration，dialysis，concentration and lyophilization，then antioxidant peptide with different molecular weights were got.