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投稿时间：2017-03-23
投稿时间：2017-03-23
中文摘要: 研究了D-苯基乳酸高产菌株在5 L发酵罐中全细胞转化苯丙酮酸钠合成D-苯基乳酸的条件，分别考察了溶氧量、表面活性剂、底物补加方式等对D-苯基乳酸产量的影响。结果表明：最适溶氧为40%，控制pH值为7.0分批补加底物得到D-苯基乳酸的产量为18.76 g/L，较未控制pH值发酵提高7.22%；添加1%吐温80时，D-苯基乳酸产量达到了18.59 g/L，比未加表面活性剂发酵提高了6.98%。在此基础上，将底物匀速流加得到D-苯基乳酸的产量提高到19.43 g/L。
Abstract:The conditions of the synthesis of D-phenyllactic acid by the whole cell transformation of phenylpyruvic acid in 5 L fermentor were studied in this paper.Dissolved oxygen，surfactant，substrate adding method on the yield of D-phenyllactic acid were studied.The results showed：the optimum dissolved oxygen was 40%，the yield of D-phenyllactic acid was 18.76 g/L when batch addition of the substrate and a constant pH of 7.0，compared with natural pH fermentation increased by 7.22%.The yield of D-phenyllactic acid was up to 18.59 g/L when 1%Twain was added，compared with that without surfactant addition increased by 6.98%.On the basis of this，the yield of D-phenyllactic acid was increased to 19.43 g/L under the uniform substrate flow.
文章编号：201721034 中图分类号： 文献标志码：
基金项目:常熟理工学院重点毕业论文团队项目
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