Abstract:A fluorescence real-time PCR method was developed to detect the Pseudomonas aernginosa，Species-specific primers and TaqManfluorescent probe were designed by Primer express 3.0 software according to the conserved sequence of Exotoxin Agene of Pseudomonas aernginosa.Different gradient concentrations of Pseudomonas aernginosa DNA and various pathogen DNA were amplified by fluorescent real-time PCR to confirm the specificity and sensitivity of the developed method.Results showed that the amplification curves were expressed from Pseudomonas aernginosa by fluorescence real-time PCR.The sensitivity of fluorescence realtime PCR for Pseudomonas aernginosa was 1×103CFU/mL.This method is valuable for research and application prospects.