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投稿时间：2017-02-19
投稿时间：2017-02-19
中文摘要: 建立应用高效液相-示差折光法同时测定米酒中葡萄糖、蔗糖和麦芽糖含量的方法。结果表明当检测器为示差折光检测器，InertSustain NH2氨基色谱柱（250 mm×4.6 mm，5 μm），流动相中乙腈和水的体积比为 75∶25，流速1.0 mL/min，进样量10 μL，检测器和色谱柱温度均为35℃时，混标及米酒样品中的3种糖在10 min内均达到基线分离，最低检测限为0.006 g/100 g，精密性试验相对标准偏差值为0.115%、0.045%和0.220%，重复性试验相对标准偏差值为0.670%、0.429%和0.723%，平均回收率为99.65%、100.85%和99.43%。由此可见，高效液相-示差折光法结果准确可靠，适用于米酒中糖的快速分析。
Abstract:The method for simultaneously determining glucose，sucrose and maltose in rice wine by high performance liquid chromatography-refractive index detector method（HPLC-RID）was established.The separation was carried out on InertSustain NH2amino column（250 mm×4.6 mm，5 μm），mobile phase was acetonitrilewater（75∶25），flow rate was 1.0 mL/min，sample size was 10 μL，and column and detector temperature was 35℃.The glucose，sucrose and maltose were simultaneous separated under the optimized conditions within 10 minutes，the detection limit was 0.006 g/100 g，the results of precision test were 0.115%，0.045%and 0.220%，the relative standard deviation（RSD）of repetitive test were 0.670%，0.429%and 0.723%，and the results of spike recovery test were 99.65%，100.85%and 99.43%.Thus，HPLC-RID with the properties of accurate and reliable，and could be applied for the rapid analysis of carbohydrate in rice wine.
keywords: high performance liquid chromatography refractive index detector rice wine glucose sucrose maltose
文章编号：201721026 中图分类号： 文献标志码：
基金项目:湖北省高等学校优秀中青年科技创新团队计划项目（T201616）
| 作者 | 单位 |
| 杨成聪1，凌霞2，胡伟伟1，张振东1，范文莹1，郭壮1，* | 1.湖北文理学院化学工程与食品科学学院鄂西北传统发酵食品研究所，湖北襄阳441053；2.襄阳市食品药品检验所，湖北襄阳441021 |
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