Abstract:Uncertainty is a class of important parameters to represent the reliability of the measurement results.It has an important significance to standardize the experimental operation.Surface enhanced Raman spectroscopy（SERS）is a molecular vibrational spectroscopy for both qualitative and semi-quantitative detection of analytes.Recently，SERS had been widely used in the food safety and quality control areas owning to its several merits of fast detection，simple sample preparation，high sensitivity，etc.The quantitative detection of melamine had been established using SERS，and the uncertainty of SERS method had been analyzed meanwhile.Melamine showed the characteristic Raman peak at 715 cm-1steadily when mixed with Au nanoparticles.And the peak intensity of 715 cm-1had a positive relationship with the concentration of melamine.Taken 675 cm-1as the internal standard peak，there was a linear regression between I715/I675and the concentration of melamine in the range of 0.1 μg/mL-5.0 μg/mL.The correlation coefficient was 0.995 4，and the detection limit reached 0.068 6 μg/mL.The major uncertainty of SERS method was calculated and which mainly arise from the establishing of the standard curve by the least square method.Uncertainty analysis of SERS method would be benefit for the comparability and traceability of the measurement results.