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投稿时间：2017-02-06
投稿时间：2017-02-06
中文摘要: 建立牛乳中未衍生游离氨基酸的超高效液相色谱串联质谱的测定方法。样品采用酸化乙腈沉淀蛋白，正己烷脱脂，氮吹至近干后初始流动相复溶，超声溶解离心过膜后检测。结果显示：在14 min内所分析目标物得到较好的分离，浓度与峰面积的线性关系良好，相关系数在0.991 2～0.999 8之间，回收率在76%～124%，相对标准偏差1.0%～12.9%。
中文关键词: 牛乳 未衍生游离氨基酸 超高效液相色谱串联质谱 亲水作用
Abstract:A method for determining of underivatized free amino acids in milk was developed with ultra-high performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS）.The protein of sample was precipitated with acid acetonitrile，the supematant was purified by n-hexane，then the purified solution was concentrated by nitrogen，dissolved with the initial mobile phase by ultrasonic assistance，centrifuged and filtered for ultra-high performance liquid chromatographic analysis.The results indicated that good separation of amino acids was obtained within 14 min.The linearity of the investigated compounds between concentrations and their peak areas within the test ranges was good，and the correlation coefficient was from 0.991 2 to 0.999 8.The average recoveries and relative standard deviations of 14 amino acids at three spiked levels were in the range of 76%-124%and 1.0%-12.9%respectively.
keywords: milk underivatized free amino acids ultra-high performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS） hydrophilic interaction
文章编号：201719034 中图分类号： 文献标志码：A
基金项目:
| 作者 | 单位 |
| 赵瑞1，崔进2，梅连瑞2，许晓菁2，金钥2，闫师杰1，3，*，刘祥4，* | 1.天津农学院食品科学与生物工程学院，天津300384；2.天津市产品质量监督检测技术研究院，天津300308；3.天津市农副产品深加工技术工程中心，天津300384；4.天津市食品安全检测技术研究院，天津300308 |
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