Abstract:Determination of arsenic inLentinus edodesby atomic fluorescence spectrometry，uncertainty components in testing processes were analyzed and recognized，evaluation of function model was established，combined standard uncertainty and expanded uncertainty were calculated.The method of uncertainty evaluation for determination of arsenic inLentinus edodeswas established.The results showed that the most affected for laboratory uncertainty was sample preparation.Therefore，the process of sample preparation should be strict in operation，the digestion conditions should be controlled carefully.This evaluation model can provide reference for the quality control adopting this method to determine the content of arsenic inLentinus edodes.