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食品研究与开发:2017,38(19):18-23
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石榴酒体外及斑马鱼体内抗氧化活性研究
张洪亚1,2,王振国3,刘邦刚3,楚杰2,*,刘可春2,杨桂文1,*
(1.山东师范大学生命科学学院,山东济南250014;2.山东省科学院生物研究所山东省生物检测技术工程实验室/山东省生物传感器重点实验室/山东省科学院药物筛选技术重点实验室,山东济南250014;3.济南大学山东省医学科学院医学与生命科学学院,山东济南250002)
Research in the Antioxidant Activity of Pomegranate Wine in vitro and Zebrafish in vivo
ZHANG Hong-ya1,2,WANG Zhen-guo3,LIU Bang-gang3,CHU Jie2,*,LIU Ke-chun2,YANG gui-wen1,*
(1.Life Sciences Institute,Shandong Normal University,Jinan 250014,Shandong,China;2.Shandong Provincial Engineering Laboratory for Biological Testing Technology/Shandong Provincial Key Laboratory of Biosensors/Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences,Institute of Biology,Shandong Academy of Sciences,Jinan 250014,Shandong,China;3.School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medicine Sciences,Jinan 250002,Shandong,China)
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投稿时间:2017-02-21    
中文摘要: 探对石榴酒的抗氧化活性进行研究,采用分光光度法测定石榴酒对DPPH·、·OH和O2-自由基的清除能力和石榴酒的还原力,并利用皮肤荧光斑马鱼进行石榴酒的抗氧化活性试验。结果表明:石榴酒在一定范围加入量内对DPPH·、·OH和O2-有良好的清除作用,清除率最大分别为92.95%、82.76%和63.85%。在对皮肤荧光斑马鱼进行石榴酒的抗氧化活性体内试验中,石榴酒浓度在30、40、50μL/mL时,相对抗氧化能力分别达到了39.7%、60.3%、75.4%。
中文关键词: 石榴酒  抗氧化活性  自由基  斑马鱼
Abstract:The study was to investigate the antioxidant activity of pomegranate wine.The antioxidant activity of the pomegranate wine was evaluated by spectrophotometry on its ability of scavenging 1-diphenyl-2-picrylhydrazy(DPPH·),hydroxyl radical(·OH),superoxide anion radical(O2-·)and total reducing power.The zebrafish with fluorescent skin was used to test the antioxidant activities of the pomegranate wine in vivo.The results showed that pomegranate wine had a well scavenging activity on DPPH·,·OH and O2-·in a certain range,and the highest scavenging activity were 92.95%,82.76%and 63.85%,respectively.In vivo experiment in zebrafish with fluorescent skin showed that the highest scavenging activity of pomegranate wine were 39.7%,60.3%and 75.4%in a certain concentration range(30,40,50 μL/mL).
文章编号:201719005     中图分类号:    文献标志码:A
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