Abstract:Selenium exists in nature in two forms：inorganic selenium and organic selenium.Mainly introduces the pretreatment method of selenium form and the different detection methods，such as fluorescence method,graphite furnace atomic absorption spectrometry，hydride atomic fluorescence,liquid chromatography，capillary electrophoresis，colorimetric method and spectroscopy.It is expected to be a rapid,accurate and easy method for the determination of organic selenium in the aspects of conductivity，enzyme activity and enzyme-linked immunoassay，and provided a theoretical basis for the development of selenium detection technology.