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投稿时间：2016-12-23
投稿时间：2016-12-23
中文摘要: 建立超高效液相色谱同时测定饮料中6种人工合成色素（柠檬黄、苋菜红、胭脂红、日落黄、诱惑红、亮蓝）的方法。饮料用聚酰胺粉吸附色素，除去杂质后用乙醇-氨水-水（7∶2∶1，体积比）洗脱色素，以甲醇-0.02 mol/L乙酸铵为流动相，梯度洗脱，在ACQUITY UPLCRBEH C18（2.1 mm×50 mm，1.7 μm）色谱柱上分离，在254 nm波长处检测。6种色素分离效果良好，线性范围宽，相关系数r≥0.999 5，实际样品加标回收率为82.2%～104.5%，相对标准偏差为0.04%～4.6%。
Abstract:An ultra-high performance liquid chromatography （UPLC）method was developed for the simultaneous determination of six kinds of synthetic pigment，including Tartrazine，Amaranth，Ponceau 4R，Sunset yellow，Allura red，Brilliant blue in beverage samples.Synthetic pigments were adsorbed by polyamide，eluted by ethanol-ammonia-water（7 ∶2 ∶1，volume ratio）after the removal of impurities，separated on an ACQUITYUPLCRBEH C18column（2.1 mm×50 mm，1.7 μm）in gradient elution mode using a mobile phase made up of methanol and 0.02mol/L ammonium acetate，and detected at 254 nm.The method had good effect in separating six kinds of pigment.The calibration curves had wide linearity range with correlation coefficient r≥0.999 5.The recoveries in spiked samples were in the range of 82.2%-104.5%and the relative standard deviations（RSD）were 0.04%-4.6%.
文章编号：201718034 中图分类号： 文献标志码：A
基金项目:
| 作者 | 单位 |
| 方晓丽 | 平阳县疾病预防控制中心，浙江平阳325400 |
| Author Name | Affiliation |
| FANG Xiao-li | Pingyang Center for Disease Control and Prevention，Pingyang 325400，Zhejiang，China |
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