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投稿时间：2017-01-10
投稿时间：2017-01-10
中文摘要: 建立一种快速、有效的高效液相色谱法，测定樱桃汁饮料中芍药素-3-葡萄糖苷、锦葵定-3-葡萄糖苷的方 法。样品经 0.5 %盐酸乙醇提取后，采用 C18 柱（4.6 mm×150 mm，5 μm）分离，以乙腈-5 %磷酸梯度洗脱的条件下，用 PDA 检测器检测，外标法峰面积定量。结果表明，芍药素-3-葡萄糖苷、锦葵定-3-葡萄糖苷标液在 20.0 μg/mL~ 200.0 μg/mL 浓度范围内线性良好，相关系数 r2 为 0.999，芍药素-3-葡萄糖苷检出限为 40 mg/kg，定量限为 120 mg/kg， 锦葵定-3-葡萄糖苷检出限为 50 mg/kg，定量限为 150 mg/kg，两个目标物的加标回收率为 88.4 ％~92.6 ％。该方法具有 前处理简单、检测速度快的优点，适用于樱桃汁饮料中芍药素-3-葡萄糖苷、锦葵定-3-葡萄糖苷的定量检测。
Abstract:A rapid and effective method was established for the determination of peonidin -3-glucoside and malvidin -3-glucoside in cherry juice drink by HPLC. After the sample was extracted by 0.5 % ethanol hy－ drochloride， the targeted compound was separated on C18 column（4.6 mm×150 mm， 5 μm） using acetonitrile -5 % phosphoric acid as mobile phase by gradient elution， with PDA detector and external standard method peak area quantification. The linear range of peonidin -3-glucoside and malvidin-3-glucoside was in the range of 20.0 μg/mL-200.0 μg/mL with a correlation coefficient of 0.999. The peonidin -3-glucoside detection limit was 40 mg/kg and the quantitative limit was 120 mg/kg. The malvidin-3-glucoside detection limit was 50 mg/kg and the quantitative limit was 150 mg/kg.The two targets recovery rate was 88.4 %-92.6 %. The method had the ad－ vantages of simple pretreatment， good extract effect and fast detection rate， and was suitable for the separation and quantitative detection of peonidin -3- glucoside and malvidin -3-glucoside in cherry juice drink.
文章编号：201717035 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| AN Chun-zhi， WANG You-zhi | NanHua College of Industry and Commerce， Guangzhou 510507， Guangdong， China |
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