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投稿时间：2017-05-05
投稿时间：2017-05-05
中文摘要: 以海藻酸钠为载体，戊二醛为交联剂，对 L-阿拉伯糖异构酶进行固定化。研究海藻酸钠浓度、氯化钙浓度、戊 二醛浓度以及硬化时间等因素对固定化效果的影响。结果表明最佳固定化条件为：海藻酸钠浓度 3.5 %，氯化钙浓度3.0 %，戊二醛浓度 0.2 %，硬化时间 7 h，该条件下制备的固定化酶与游离酶相比，最适宜反应温度和 pH 值基本没有 变化，并且具有较好的操作稳定性和贮存稳定性。
Abstract:L-alabinose isomerase （L-AI）was immobilized using sodium alginate as the carrier and flutaralde－ hyde as the crosslinker. The effects of the concentration of sodium alginate， calcium chloride and flutaralde－ hyde， the solidifying time were studied. The results showed that the optimal immobilization condition were as followed： sodium alginate 3.5 %， calcium chloride 3.0 %， flutaraldehyde 0.2 % and the solidifying time 7 h. Under the optimum condition， compared with the free L-AI， the optimum pH value and temperature of immo－ bilized L-AI were not changed. The immobilized L-AI had good operational stability and storage stability.
文章编号：201716020 中图分类号： 文献标志码：A
基金项目:天津市科技计划项目（15YFFCTG00130）
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