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投稿时间：2016-10-21
投稿时间：2016-10-21
中文摘要: 研究大孔吸附树脂法纯化分离原花青素的工艺条件及参数。采用香草醛-硫酸方法进行分光光度法测定，考 察 AB-8 大孔树脂对原花青素的吸附率、吸附量、解吸附率、纯度。结果表明：上样液中原花青素含量在 4.5 mg/mL~6.5 mg/mL 范围较为合适，当 AB-8 树脂与提取液的比例为 1∶10（g/mL），上样流速为 1.5 BV/h，吸附时间为 8 h 时，吸附 率达到 95 %以上；以 40 %乙醇溶液为洗脱剂，洗脱剂用量为 6 BV，解析率为 99 %，此条件下得到的固体纯度为94.7 %，用 AB-8 型大孔树脂分离，纯化原花青素的方法可行。
Abstract:To optimize the technical parameters of optimize the technical parameters of procyanidins separation and purification with macroporous resin. The vanillin-sulfuric acid spectrophotometry was used to investigate adsorption rate， adsorbtion capacity， eluting rate and purity of procyanidin. The results indicated that the opti－ mum ranges of procyanidin in sample solution was 4.5 mg/mL to 6.5 mg/mL.The rate of AB-8 resin and reagent was 1∶10 （g/mL），the flow rate was 1.5 BV/h and the adsorbtion time was 8 h，the adsorbtion capacity of pro－ cyanidin was more than 95 %. The eluting rate of procyanidin and the purity of solid were 99 % and 94.7 %， re－ spectively， by using 40 % ethyl alcohol as eluent with 6 BV of dosage. The macroporous adsorption resin method is reliable and can be applied for separation and purification of procyanidin.
keywords: macroporous resin grape seed procyanidins purification
文章编号：201716012 中图分类号： 文献标志码：A
基金项目:新疆生产建设兵团中青年科技创新领军人才（2015BC001）
| Author Name | Affiliation |
| MA Xiao-qin，WANG Xiao-min， ZHOU Fan* | Xinjiang Hua Shidan Pharmaceutical Reaearch Co.，Ltd.，Urumqi 830011，Xinjiang，China |
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