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食品研究与开发:2017,38(15):50-53
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酶法提取天麻多糖工艺优化
谭沙,朱仁威,张静,李刚凤,李丽,邹婷
(铜仁学院 材料与化学工程学院, 贵州 铜仁 554300)
Extraction Process Optimization of Polysaccharide in Gastonia elate by Enzymatic Method
TAN Sha, ZHU Ren-wei, ZHANG Jing, LI Gang-feng ,LI Li, ZOU Ting
(School of Material and Chemical Engineering, Tongren College, Tongren 554300, Guizhou,China)
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投稿时间:2017-03-19    
中文摘要: 对影响天麻多糖提取率的酶用量、提取时间、提取温度及 pH 值进行单因素试验,并使用正交法得到酶法提 取天麻多糖最佳工艺。结果表明,提取天麻多糖最佳工艺为酶用量 8 mg/g,提取时间 110 min,提取温度为 40 ℃,pH 值 为 4.5,在最优提取工艺条件下,天麻多糖提取量为 46.63 mg/g。
中文关键词: 天麻  酶解法  正交试验  多糖
Abstract:Influencing factors which affected the extraction yield of polysaccharide in Gastonia elate was stud- ied, including enzyme amount , temperature, time, and pH, and the extraction conditions of polysaccharide in Gastonia elate were optimized by orthogonal experiments. Result as follows: the enzyme amount was 8 mg/g, the enzymatic hydrolysis time was 110 min, the enzymatic extraction temperature was 40 ℃, the pH was 4.5, and the polysaccharide in Gastonia elate was 46.63 mg/g under the optimum conditions.
文章编号:201715011     中图分类号:    文献标志码:A
基金项目:铜仁市科技局计划项目(铜市科研[2014]40-6);铜仁学院食品科学与工程校级重点支持学科建设项目(0002);贵州省教育厅大学生创新创业训练中心项目(2015SJDCZX001);贵州省教育厅资助(黔学位办[2013]18 号)
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