Abstract:Compared the contents of catalpol， verbascoside ， rehmaionoside D and leonuride in rehmanniae flowers， for the flower's medicinal and edible aspects provide certain reference.Contents of catalpol，verbasco－ side ，rehmaionoside D and leonuride were determined by HPLC，Waters -Symmetry ShieldTM RP18 column （4.6 mm × 250 mm，5 μm）， catalpol：a mobile phase of acetonitrile- water （1 ∶ 99 ） was used，flow rate was 1 mL/min，column temperature was set at 30 ℃ ，a UV detector was applied with wavelength of 210 nm，sample volume10μL.Verbascoside：a mobile phase of acetonitrile - 0.1 % acetic acid solution （18 ∶ 82） as mobile phase，flow rate was 1 mL/min，column temperature was set at 30 ℃ ，a UV detector was applied with wavelength of 334 nm， sample volume 20 μL. Rehmaionoside D and leonuride： SunFireTM C18 column（4.6 mm × 250 mm， 5 μm）， a mobile phase of acetonitrile- water （3 ∶ 97） was used，flow rate was 1 mL/min，column temperature was set at 30 ℃ ，a UV detector was applied with wavelength of 203 nm，sample volume 20 μL. The content of catalpol content： petals > pedicel > receptacle， as high as 9.52 %； verbascoside content： petals > receptacle > peduncle， up to 0.53 %； rehmaionoside D levels： petals > pedicel > receptacle， as high as 0.09 %； leonuride content： receptacle > pedicel > petals， as high as 0.19 %. There are significant differences among different parts of the active ingredient content of rehmanniae flowers.