Abstract:Trichosanthes seed oil is rich in unsaturated fatty acid composition. The refining can improve its edi－ ble quality and commodity value. The low temperature cold pressing method was used to prepare the seed oil. Basis on the analysis of the basic indexes of the seeds oil， the refining process of the seeds oil was also studied. The results showed that the seed oil extracted by the cold pressing method could retain more nutritive materials， and to ensure the extraction of linolenic acid， the seeds needed to be fried before pressing. The content of polyunsaturated fatty acids in trichosanthes seed oil was 65.91 %， which included the characteristic fatty acids of trichosanthes seed oil was 27.24 %. After refining， the moisture， acid value and peroxide value of the tri－ chosanthes seed oil had obviously decreased， which indicated that the refining process not only was ideal， but also improved the quality of trichosanthes seed oil， and more conducive to storage.