Abstract:This research studied the contents of alkaloids， flavonoids， sterol as well as volatile oil from the Plumula nelumbinis of the five origins of Jian Ning， Guang Chang， Bao Ying， Hu Nan， Hong Hu. With ethanol as extraction solvent， we extracted physiologically active substances from Plumula nelumbinis powders by ultra－ sonic extraction way， and measured with spectrophotometry. The highest content of alkaloids in Plumula nelumbinis was Guang Chang Lotus 7.18 mg/g. The highest content of flavonoids in Plumula nelumbinis was Hong Hu Lotus 14.28 mg/g. Extract by organic solvents， saponification， solid phase extraction， derivation， and nitrogen blow， and then detect the content of sterol in the Plumula nelumbinis by Gas Chromatography-Mass Spectrometer. The highest content of sterol in Plumula nelumbinis was Bao Ying Lotus 0.40 mg/g， and the main kind of sterol was β-sitosterol. Extract the volatile oil by steam distillation， and detect it by Gas Chromatogra－ phy-Mass Spectrometer. The highest content of volatile oil in Plumula nelumbinis was Hu Nan Lotus 0.60 %. The content of linoleic acid and linoleic acid esters， palmitic acid and palmitic acid ester played a major part， and the content of phytol， phenols， and squalene accounted for a minor place.This article established the database and conducts functional evaluation of Plumula nelumbinis of different areas for further study of Plumula nelumbinis resource， by detecting the contents of alkaloids， flavonoids， sterols and volatile oil of Plumula nelumbinis in five origins. Guang Chang Plumula nelumbinis had a relative high functional ingredients， it is promising to develop and use Guang Chang Plumula nelumbinis resource.