Abstract:A rapid and effective method was established for the determination of trace imidaeloprid pesticide residue in fruit and vegetable drink by ultra performance liquid chromatography tandem mass spectrometry （UHPLC-MS/MS）. After the sample was extracted by 0.1 % acetic acid-acetonitrile， it concentrated and puri－ fied by amino solid phase extraction column. The separation of targeted compound was performed on a Waters Atalantis T3 chromatographic column（2.1 mm×150 mm， 3 μm） using 0.1 % formic acid-acetonitrile as mobile phase under gradient elution mode. The linear range of imidaeloprid was in the range of 0.5 ng/mL-50.0 ng/mL with a correlation coefficient of 0.999. The detection limit was 0.15 μg/kg and the quantitative limit was 0.5 μg/kg. The recovery rate was 93.2 %-96.9 %.