Abstract:The single factor and response surface method was used to optimize the ultrasonic extraction of flavonoids from Aronia melanocarpa. The anti-fatigue effect of the flavonoids of Aronia melanocarpa was studied by mouse experiment. The results showed that the optimal extraction parameters was ultrasound power 70 W， ul－ trasonic temperature 61 ℃， and ultrasonic time 124 min. The extraction rate of flavonoids was 95.64 %. The fac－ tors influencing the extraction rate of flavonoids from Aronia melanocarpa was ultrasonic temperature> ultrasonic time> ultrasonic power from strong to weak. After mice fed with flavonoids， the exhaustive swimming time of the mice was significantly prolonged and the higher the concentration the more obvious，the blood lactic acid content was compared with that of the non-perfused flavonoids group. Flavonoids of Aronia melanocarpa on mice had strong anti-exercise fatigue function.