Abstract:Stable Isotope fingerprint technology had the advantage of high sensitivity， simple experimental op－ eration. It could be also used to distinguish the tracking material whether it was a new addition or it was inherent to the test system，etc.It had been widely used in the origin of agricultural products traceability， it was of great significance to distinguish agricultural products from different regions of China. This paper mainly expounded the basic principles of the isotope fingerprinting technology and its advantages and limitations in the agricultural product origin traceability， the application situation of the origin of agricultural products was briefly introduced and the prospect was put forward.