Abstract:This research studied the contents of alkaloids， flavonoids， sterol as well as volatile oil from the lotus leaves of the five famous origins of lotus in China of Guangchang Lotus （Jiangxi Guangchang）， Jiangsu Hong Lotus（Jiangsu Baoying）， Hong Hu Lotus（Hubei Honghu）， Xiang Lotus（Hu'nan） and Jian Lotus（Jianning， Fujian）. With ethanol as extraction solvent ， physiologically active substances was extracted from lotus leaves powder by ultrasonic extraction way， and measured with spectrophotometry. The highest content of alkaloids in lotus leaves was Xiang Lotus 4.08 mg/g. The highest content of flavonoids in lotus leaves was BaoYing Hong Lo－ tus 19.99 mg/g. Ethanolic extract from lotus leaves was carried on with saponification， solid phase extraction， derivation， and nitrogen blow， and after that the content of sterol in lotus leaves was detected by Gas Chro matography-Mass Spectrometer. The highest content of sterol in lotus leaves was Bao Ying Hong Lotus 0.25 mg/g， and the main kind of sterol was β-sitosterol. The volatile oil was extracted by steam distillation， and its content was detected by Gas Chromatography-Mass Spectrometer. The highest content of volatile oil in lotus leaves was Hong Hu Lotus 0.75 %. The content of phytol and olefin played a major part in volatile oil of lotus leaves.