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投稿时间：2016-09-23
投稿时间：2016-09-23
中文摘要: 建立一种简便、重复性好的高效液相色谱可见光测定雪糕中的柠檬黄、苋菜红、胭脂红、日落黄、诱惑红、亮 蓝 6 种合成着色剂。以无水乙醇-氨水-水（7 ∶ 2 ∶ 1，体积比）为溶剂提取，HLB 固相萃取柱净化，ZORBAX SB-C18（4.6 mm×250 mm，5 μm），流动相以甲醇和 20 mmol/L 乙酸铵进行梯度洗脱。流速 1.0 mL/min，柱温 25 ℃，多波长同时测定 6 种 的合成色素，检测波长分别为 428、521、509、483、507、625 nm。结果表明：6 种合成色素均达到基线分离，在 0.1 μg/mL~ 10 μg/mL 范围内，具有良好的线性关系（R2 均大于 0．999 9）；样品的样品加标回收率 85 %~110 %，精密度 RSD 均小于2.0 %。该方法可用于检测雪糕中合成色素的含量。
Abstract:To develop a method for the determination of six synthetic pigments such as tartrazine， amaranth， carmine， sunset yellow， temptation red， brilliant blue， fancy red in ice cream. Using anhydrous ethanol-am－ monia-water（7 ∶ 2 ∶ 1，V/V） as solvent extraction， the synthetic pigments were cleaned by HLB cartridge. After exreacetd， samples were separated in a ZORBAX SB-C18 column（4.6 mm×250 mm，5 μm）column with gradient program by using methanol and 20 mmol/L ammonium acetate as mobile phase at a flow rate of 1.0 mL/min， with the column temperature 40 ℃ and detection wave 428，521，509，483，507，625 nm. The six synthetic pigments were separated. The linear range of menthod was in the range of 0.1 μg/mL -10 μg/mL：（R2>0.999 9）.The re－ coveries for samples ranged from 85 %-110 % ，and RSD were all below 2 %. This methods were suitable for determination of synthetic colout in ice cream.
文章编号：201712032 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| YOU Yan | The Inspection Testing Center of Food and Drug in YuHuan， Taizhou 317608， Zhejiang， China |
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