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食品研究与开发:2017,38(12):13-16
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紫玉米苞叶花色苷与牛血清白蛋白相互作用研究
朱莉,马越,赵晓燕,王丹*
(北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,农业部都 市农业(北方)重点实验室,北京 100097)
Study on the Interaction between Purple Corn Bract Anthocyanins and Bovine Serum Albumin
ZHU Li, MA Yue, ZHAO Xiao-yan, WANG Dan*
(Beijing Academy of Agriculture and Forestry Vegetable Research Center , Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China), Ministry of Agriculture, P.R.China, Key Laboratory of Urban Agriculture(North), Ministry of Agriculture, P.R.China, Beijing 100097, China)
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投稿时间:2016-09-28    
中文摘要: 采用荧光光谱法、紫外可见吸收光谱法、高效液相色谱法研究紫玉米苞叶花色苷与牛血清白蛋白(BSA)之间的 相互作用。结果表明,紫玉米苞叶花色苷与 BSA 之间存在一定的相互作用,结合程度随着紫玉米苞叶花色苷浓度的增 加而增加,二者结合后,会引起 BSA 结构的变化。紫玉米苞叶中 6 种主要的花色苷成分与 BSA 结合程度不同,其结合能 力大小依次为:天竺葵-3-(6' -丙二酰葡萄糖苷)>芍药-3-(6'-丙二酰葡萄糖苷)>天竺葵-3-葡萄糖苷>矢车菊-3-葡萄 糖苷 > 矢车菊-3-(6'-丙二酰葡萄糖苷)>芍药-3-葡萄糖苷。
Abstract:Fluorescence spectrometry, UV-VIS absorption spectroscopy, and high-performance liquid chro- matography (HPLC) were used to research the interaction between purple corn bract anthocyanins and bovine serum albumin (BSA). The results showed that there was a relationship on the purple corn anthocyanins bract and BSA. The bonding degree increased with the increase of the concentration of anthocyanins . Furthermore, the structure of BSA was changed after the combination. The binding abilities of six anthocyanins in purple corn bract was different. The order of combining ability was as follows: pelargonidin-3-(6' -malonylglucoside)>pe- onidin-3-(6'-malonylglucoside)>pelargonidin-3-glucoside>cyanidin-3-glucoside>cyanidin-3-(6'-malonyl- glucoside)>peonidin-3-glucoside.
文章编号:201712004     中图分类号:    文献标志码:A
基金项目:北京市农林科学院青年科研基金(QNJJ201603); 北京市 科技新星计划(Z131105000413023)
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