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投稿时间：2016-09-07
投稿时间：2016-09-07
中文摘要: 以菊芋菊粉液为原料，采用内切型菊粉酶水解法生产低聚果糖。在单因素的基础上，选取加酶量、温度、时 间、pH 值为自变量，以转化率为响应值，通过 Box-Behnken 响应面设计对其酶解工艺参数进行优化。其最优工艺为： 加酶量 2.5 U/g，酶解时间 6.0 h，酶解温度 60 ℃，酶解 pH 值为 6.0 的条件下，低聚果糖的转化率可达到 96.27 %，与预 测值之间的相对误差为 1.91 %。
Abstract:Fructo-oligosaccharide was prepared from extraction of Jerusalem artichoke as a inulinase immobi－ lization hydrolysis method. Four enzymolysis parameters including enzyme dosage， enzymolysis temperature， enzymolysis time and enzymolysis pH were optimized as the result of the conditions for enzymolysis of fructooligosaccharide from Jerusalem artichoke by Box-Behnken Response surface design methodology based on the percent conversion as a response value. Results showed that the optimum condition of enzymolysis was as fol－ lows： the dosage of enzyme 2.5 U/g， enzymolysis time 6.0 h， enzymolysis temperature 60 ℃， and enzymolysis pH6.0. Under this condition fructo-oligosaccharide percent conversion was up to 96.27 %. Compared with pre－ dicted values and the relative error was 1.91 %.
keywords: jerusalem artichoke fructo-oligosaccharide inulinase percent conversion response surface design
文章编号：201711024 中图分类号： 文献标志码：A
基金项目:甘肃省技术研究与开发专项计划（1207TCYA039）
| Author Name | Affiliation |
| CHEN Xing-du， ZHAI Dan-yun， CHEN Qing-an， ZHAO Li-li， DONG Yan-hu， LI Yun， NIU Jie | Gansu Institute of Business and Technology， Lanzhou 730010， Gansu， China |
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