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投稿时间：2016-09-21
投稿时间：2016-09-21
中文摘要: 采用间歇式超声辅助提取金针菇菇根多糖，通过单因素试验和响应面试验设计优化金针菇菇根多糖提取工艺 条件。结果表明，影响金针菇菇根多糖得率的工艺因素按主次顺序排列为后段超声时间（C）＞恒温提取时间（B）＞前段超 声时间（A）。确定金针菇菇根多糖最佳提取工艺条件为提取液 pH5，料液比 1 ∶ 30（g/mL），提取温度 90 ℃，超声功率 400 W，前段超声时间 20 min，恒温提取时间 130 min，后段超声时间 23 min，在此最佳条件下，多糖得率为 7.24 %。在超 声时间和恒温提取时间相同条件下，该方式多糖得率比“前超声辅提+恒温提取”方式和“恒温提取+后超声辅提”方式分 别提高 6.3 %和 5.6 %，比直接恒温水浴提取提高 12.76 % 。
Abstract:Polysaccharide in base of Flammulina velutipes root was extracted with the assistance of intermittent ultrasonic. By single factor experiment and response surface design in optimizing the Flammulina velutipes root polysaccharides extraction conditions. The results showed， polysaccharide yield was most significantly affected by extraction after ultrasonic time，followed by thermostat extraction time and front ultrasonic time. An optimum polysaccharide yield of 7.24 %（on the basis of Flammulina velutipes root powder）were obtained when Flam－ mulina velutipes root was treated with pH5，solid-liquid ratio 1 ∶ 30 （g/mL）， extraction temperature 90 ℃， ul－ trasonic power 400 W， front ultrasonic time 20 min， thermostat extraction time 130 min， after ultrasonic time 23 min. In the ultrasonic extraction time and thermostat extraction time under the same conditions，the polysac charide yield of the mode respectively 6.3 %， 5.6 %，12.76 % than the "front ultrasound + thermostat extraction" mode，the "thermostat extraction + after ultrasound" mode， the "direct thermostatic water bath extraction"mode.
keywords: Flammulina velutipes root polysaccharide intermittent ultrasonic assisted extraction response surface
文章编号：201711009 中图分类号： 文献标志码：A
基金项目:泰山产业领军人才项目（LJNY2015004）
| Author Name | Affiliation |
| ZHANG Ming， LI Xin-sheng*， MA Chao，WANG Chao-chuan， MENG Xiao-feng | Jinan Fruit Research Institute， China Supply and Marketing Cooperatives， Jinan 250014， Shandong， China |
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