Abstract:This study took beef longissimus dorsi as material， dealing with air-packaging（AP）， vacuum pack－ aging（VP） and modified atmosphere packaging（MAP）， in which the MAP were divided into two forms： MAP1 for high oxygen packaging（78.8 %O2/18.8 %CO2/2.4 %N2） and MAP2 for no oxygen packaging（60 %CO2/40 % N2）. They were stored at -1.5 ℃ and 2 ℃ separately for retaining freshness， the index of microorganisms（total visible counts and psychrophilic counts） and biogenic amine were studied and the correlation between microbial and biogenic amine formation were analyzed.The result showed that the total visible counts and psychrophilic counts increased in all samples with different packaging forms and storage temperature. AP group in 10 days， the total visible counts and psychrophilic counts were more than 6.00 lgcfu/g， but VP and MAP could inhibit the growth of microorganisms.A high-performance liquid chromatographic hyphenated was developed for deter－ mination of eight kinds of biogenic amines（tryptamine， phenylethylamine， putrescine， cadaverine， histamine， tyramine， spermidine， spermine） in four kinds of packaging forms of beef during storage. The results showed that the amount of biogenic amines in different treatment group increased during storage， putrescine were the main biogenic amine， cadaverine and tyramine followed， typtamine， phenethylamine and hisamine were not checked out. There was a significant positive correlation between formation of biogenic amine and the growth of microorganism through the correlation analysis. Among them， the putrescine and total biogenic amine could be used for the deterioration index of beef because there was a significant level （P＜0.01）.